Lab Alumni:

Chris Chamblee, Andrew Smith*, Tyler Sodia*, Lindsey Armstrong, Austin Haider, Marcos Maldonado*, Anika James, Marlea Kudlauskas, Lisa Fetter*, Anna Nguyen*, Derek Clark, Ilia Mazin, Nazar Dubchak, Jena Jacobs, Jessica Daniel*, Aviva Bulow, Susan Jett*, Ryan Warren, Tiffany Ashbaugh, Michael McCoy, Ebony Miller, Jonathan Richards*, Laura Roon*, Becky Addison, Jeremy O’Brien, Travis Ingraham, Sarai Graves, Kathryn Norquest*, Stephen Schaffner*, Kyra Brandt, Elina Baravik*, Yerelsy Reyna*, Josh Sowick, Jody Stephens*, Ryan Masterson*, Mason Preusser, Tonya Santaus, Amanda Faux, Matthew Stoddard, Morgan Miller.
(* denotes a researcher with a publication from the lab)


Dr. Andrew J. Bonham


Professor of Chemistry & Biochemistry
Dr. Bonham’s Curriculum Vitae

Dr. Bonham’s work focuses on understanding and investigating Transcription Factors, essential human proteins that regulate the bodies growth and response to disease. These transcription factors are essential components of gene regulation, and there is great interest in probing their presence and activity in both academic analysis and clinical diagnostics. Current methods to address these questions are often time-intensive or require specialized reagents, such as antibodies. At Metropolitan State University of Denver, Dr. Bonham is leading an innovative undergraduate research program focused on engineering new tools for sensitive and quick detection of TF:DNA interactions.


Dylan Poch



Lab Member 2019-
Project: Electrochemical DNA Biosensors for Detection of Mannose-capped Lipoarabinomannan
Published!

Mycobacterium Tuberculosis (TB) is one of the world’s most prevalent bacterial pathogens. It is estimated that almost 10 million cases of TB emerge every year, and roughly one-fifth of these cases are fatal. The current detection and diagnosis of TB is done primarily via two methods; the TB skin test and the TB blood tests. Neither of these tests can differentiate between latent TB infection and TB disease. In order to differentiate these states, time-consuming sputum tests are required, which rely on culturing the mycobacterium. Designing a sensitive serologic biosensor would dramatically decrease the time line of diagnosis and therefore improve patient outcomes. One possible avenue for improved detection lies in the cell wall of TB, which includes many complex glycolipids—many of which are believed to have immunopathogenic mechanisms in physiologic pathways. Mannose-capped lipoarabinomannan (ManLAM) is one of the most prevalent of these glycolipids, and presents a novel target as a bio-marker for the sensitive detection of TB and related Mycobacterium strains. Here, we have utilized an existing aptamer sequence that binds to ManLAM to generate a sensitive electrochemical, DNA-based biosensor for the detection of TB. This biosensor is able to adopt multiple different folded conformations, only one of which presents the core aptamer sequence in a state capable of binding ManLAM. An appended redox-active tag (methylene blue) generates a measurable difference in electrochemical current upon this conformational change, providing a sensitive and quantitative measurement of ManLAM concentration. Such biosensors may ultimately allow rapid, on site, diagnosis of TB infection within the time constraints of patient-doctor interaction.


Marissa Allen



Lab Member 2020-
Project: Design of electrochemical biosensors for Whooping Cough

Whooping cough caused by Bordetella pertussis can cause serious and prolonged health effects, particularly in infants. Quick and accurate diagnosis is essential in treating the infection. Early treatment can significantly reduce the duration of illness in the patient and lead to a milder case of illness overall. The current standard for testing set forth by the CDC is invasive, requiring nasopharyngeal swabs. Most medical facilities are not equipped to then process the sample collected, and it is instead sent out for analysis adding to the length of time required to get a diagnosis for the patient. Common laboratory tests for the detection of B. pertussis are PCR and culture, but each present unique challenges. The purpose of our research is to design and develop a rapid electrochemical biosensor that can detect P.69 pertactin. P.69 pertactin is a surface protein used by B. pertussis to bind to mammalian cells. A biosensor that can detect P.69 pertactin would improve several major issues associated with the current testing standards including size of the patient sample needed, time to get results, cost of testing and shipping, and accuracy of tests. The B. pertussis biosensor will be developed through the SELEX process and will create a specific oligonucleotide probe for P.69 pertactin . This probe will then be adapted into an electrochemical DNA-based biosensor. This biosensor would require a much smaller sample from the patient and give almost immediate results to the physician overseeing the case.


Mary Quansah



Lab Member 2021-
Project: Electrochemical Biosensors for the cancer biomarker ENOX-2

Human Ecto-NOX Disulfide-Thiol Exchanger 2 (ENOX2) is a cell-surface metalocatalyst which is expressed solely on malignancies of all major human cancer types. ENOX2, along with its ubiquitous parent protein ENOX1, serve as terminal oxidases in plasma membrane electron transport chains, suggesting that such proteins are essential for cell growth. ENOX2 is frequently shed from malignancies into the sera and demonstrates site-specific transcript variants, and thus presents an attractive target for both diagnostic blood testing and anti-cancer therapeutic treatments. To better facilitate detection and targeting of ENOX2 in patient sera, we utilized SELEX to generate aptamers against recombinantly expressed ENOX2. These aptamers were characterized via gel mobility shift assays to determine the candidate aptamer with highest affinity for human ENOX2. The candidate with highest affinity was incorporated into an electrochemical DNA-based biosensor, where changes in DNA confirmation upon target binding result in changed dynamics of an appended redox reporter molecule, ultimately generating a rapid and quantitative change in the current of the electrochemical response. This novel ENOX2-targeting electrochemical biosensor allows rapid, dose-responsive readout for the presence of ENOX2 in both buffer and blood serum.